Regulatory

Part:BBa_K3511008:Design

Designed by: Yung Mau SO   Group: iGEM20_Hong_Kong_CityU   (2020-10-02)


C62-T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutant T7 promoter is used to increase the gene expression of the recombinant proteins.


Source

T7 phage, sequence found in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592457/

References

Paul, S., Stang, A., Lennartz, K., Tenbusch, M., & Überla, K. (2013, January 7). Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution. Retrieved September 30, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592457/