Regulatory
Part:BBa_K3511008:Design
Designed by: Yung Mau SO Group: iGEM20_Hong_Kong_CityU (2020-10-02)
C62-T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutant T7 promoter is used to increase the gene expression of the recombinant proteins.
Source
T7 phage, sequence found in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592457/
References
Paul, S., Stang, A., Lennartz, K., Tenbusch, M., & Überla, K. (2013, January 7). Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution. Retrieved September 30, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592457/